ANALYTICAL TECHNIQUES FOR ANIMAL PRODUCTION II

1. Explain the principles and applications of artificial insemination in animal breeding.
2. Evaluate semen quality and apply appropriate storage techniques.
3. Conduct and interpret digestibility trials using in vivo and in vitro methods.
4. Perform biological assays to assess the nutritional value of feedstuffs.
5. Apply chromatographic and electrophoretic techniques for protein separation and characterization.
6. Utilize radio-isotopy, manometry, spectrometry, and colorimetry in biological analyses.
7. Prepare and purify enzymes for various applications.

13 weeks, about 26 hours in total. Yours will differ.

1. Week 1MODULE ONE: ARTIFICIAL INSEMINATION TECHNIQUES IN ANIMAL BREEDING

   • Unit 1: Meaning of AI and Significance of AI · 2 hours

     Define artificial insemination (AI).. Differentiate between natural mating and AI.. Understand the importance of AI in animal breeding.. Identify advantages and limitations of AI..

2. Week 2MODULE ONE: ARTIFICIAL INSEMINATION TECHNIQUES IN ANIMAL BREEDING

   • Unit 2: Semen collection techniques in farm animals · 2 hours

     Understand factors enhancing semen collection.. Learn methods of semen collection in farm animals.. Learn how to analyze semen on the farm and in the lab.. Understand the Aspiration, Massage, Electroejaculator, Gloved-hand, and Artificial vagina techniques..

3. Week 3MODULE ONE: ARTIFICIAL INSEMINATION TECHNIQUES IN ANIMAL BREEDING

   • Unit 3: Evaluation of semen quality · 2 hours

     Identify physical variables for semen evaluation: color, volume, motility.. Learn to evaluate semen quality using microscopic techniques.. Understand the importance of sperm morphology and concentration.. Perform live/dead sperm ratio tests..

4. Week 4MODULE ONE: ARTIFICIAL INSEMINATION TECHNIQUES IN ANIMAL BREEDING

   • Unit 4 Techniques involved in the storage of collected semen · 2 hours

     Learn methods for semen storage: short-term and long-term.. Identify common semen diluents and their functions.. Understand factors influencing sperm cell survival during storage.. Explore types of storage methods for frozen semen..

5. Week 5MODULE ONE: ARTIFICIAL INSEMINATION TECHNIQUES IN ANIMAL BREEDING

   • Unit 5: Semen extension, insemination and factors that may affect fertility after insemination · 2 hours

     Understand reasons for extending semen samples.. Learn about common semen diluents and their characteristics.. Study the art of insemination techniques.. Explore factors influencing fertility during insemination..

6. Week 6Module 2: Digestibility trials (*in vivo* and *in vitro*)

   • Unit 6: An overview of digestibility trials · 2 hours

     Define digestibility and digestibility trials.. Understand the significance of digestibility trials.. Differentiate between in vivo and in vitro digestibility.. Compare merits and limitations of in vivo and in vitro trials..

7. Week 7Module 2: Digestibility trials (*in vivo* and *in vitro*)

   • Unit 7: Protocols for *in vivo* digestibility trials · 2 hours

     Understand techniques involved in conducting in vivo digestibility trials.. Learn about animal protocols and diet preparations.. Explore methods of in vivo digestibility: direct collection, difference method, regression method.. Calculate digestibility parameters..

8. Week 8Module 2: Digestibility trials (*in vivo* and *in vitro*)

   • Unit 8: Protocols for *in vitro* digestibility trials · 2 hours

     Understand techniques involved in conducting in vitro digestibility trials.. Learn about Rumen fluid-pepsin in vitro digestibility (IVOMD).. Study Rumen fluid-Neutral detergent methods.. Explore Enzyme-based assays..

9. Week 9Module 3: Biological assays involving PER, NPU, AD, ID and BV

   • Unit 9: Practical applications of biological assay · 2 hours

     Understand the significance of biological assays.. Explore types of biological assays: PER, NPU, AD, TD, BV.. Learn to measure and observe livestock response in feeding experiments.. Calculate protein efficiency ratio (PER)..

10. Week 10Module 4: Separation and characterisation of components involving the use of chromatography, electrophoresis, radio-isotopy, manometry, colorimetry, spectrometry

    • Unit 10: Techniques involved in separation and characterisation of components involving the use of chromatography and electrophoresis · 2 hours

      Understand techniques for separation of proteins.. Learn about chromatography: column, HPLC, adsorption, ion exchange, hydrophobic, gel filtration, affinity.. Study separation of proteins using electrophoresis: agarose gel, polyacrylamide gel.. Understand the principles of each technique..

11. Week 11Module 4: Separation and characterisation of components involving the use of chromatography, electrophoresis, radio-isotopy, manometry, colorimetry, spectrometry

    • Unit 11: Techniques involved in separation and characterisation of components involving the use of radio-isotopy and manometry · 2 hours

      Understand the principle/application of radioisotopy to biological experiments.. Learn about the use of radioisotopy in agriculture.. Understand the principle/application of manometry to biological experiments.. Explore tracer techniques and autoradiography..

12. Week 12Module 4: Separation and characterisation of components involving the use of chromatography, electrophoresis, radio-isotopy, manometry, colorimetry, spectrometry

    • Unit 12: Techniques involved in separation and characterisation of components involving the use of spectrometry and colorimetry · 2 hours

      Understand the principle/application of spectrometry to biological experiments.. Learn about the principles and applications of colorimetry.. Explore mass spectrometry and its components.. Study the Beer-Lambert law and its application in colorimetry..

13. Week 13Module 4: Separation and characterisation of components involving the use of chromatography, electrophoresis, radio-isotopy, manometry, colorimetry, spectrometry

    • Unit 13: Determination, preparation and purification of enzymes · 2 hours

      Understand the properties and activities of enzymes.. Learn the process of extracting enzymes from tissues.. Understand the process of purifying enzymes from crude extract.. Explore the mode of action of enzymes..