General Biochemistry Practical Manual
- Health Sciences
- 200 level
- 1 credit unit
- 74 pages
- 9 units
This course provides Environmental Health Science students with practical knowledge of biochemistry. It covers pH determination, qualitative and quantitative tests for biomolecules like carbohydrates, amino acids, proteins, lipids, and vitamins. Students will also learn chromatographic separation techniques such as paper and thin layer chromatography, enabling them to perform essential biochemical analyses in the laboratory.
About this course
- Difficulty
- Intermediate
- Study hours
- 48 hours
- Maths
- Intermediate
- Content
- Practical, problem solving
- Practical work
- Yes
- Assignments
- Tutor marked assessments
- Final examination
What you'll read
The real module and unit structure of EHS209, taken from the course material NOUN publishes.
One paragraph, so you can see how it reads
EHS209 · UNIT 1: Measurement of pH of solutions
The pH scale is used to rank solutions as acidic or basic (alkaline). The pH scale ranges from 0 to 14. A pH below 7.0 is acidic, above 7.0 is alkaline, or basic while pH of 7 is considered neutral.
What you should be able to do
- Measure the pH of solutions using various methods.
- Perform qualitative tests to identify carbohydrates, amino acids, and lipids.
- Conduct quantitative estimations of carbohydrates, proteins, lipids, and vitamins.
- Apply paper and thin layer chromatography techniques for separation and analysis.
- Interpret experimental results and draw conclusions based on biochemical principles.
What it prepares you for
- Environmental Health Officer
- Laboratory Technician
- Food Quality Control Analyst
- Biomedical Scientist
- Food Industry
- Pharmaceutical Industry
- Environmental Monitoring
- Clinical Laboratories
Where it gets hard
The units students slow down on, and what makes each one heavy.
- Module 2: Quantitative Analysis and Chromatography
Unit 2: Quantitative Estimation of Proteins
The Lowry method involves multiple reagent preparations and precise timing, making it prone to errors if not carefully executed.
- Module 2: Quantitative Analysis and Chromatography
Unit 3: Quantitative Estimation of Lipids
Calculating the iodine number requires careful measurements and understanding of the chemical reactions involved.
A suggested way through it
13 weeks, about 28 hours in total. Yours will differ.
- Week 1Module 1: pH Measurement and Qualitative Test
Unit 1: Measurement of pH of solutions · 2 hours
Understand the pH scale and its significance.. Learn to use pH paper and pH meters for accurate measurements.. Conduct experiments to determine the pH of various solutions..
- Week 2Module 1: pH Measurement and Qualitative Test
Unit 2: Qualitative Test for Carbohydrates · 2 hours
Perform Molisch's test to detect the presence of carbohydrates.. Conduct Fehling's test to identify reducing sugars.. Use Barfoed's test to differentiate between monosaccharides and disaccharides..
- Week 3Module 1: pH Measurement and Qualitative Test
Unit 3: Qualitative Test for Amino Acid · 2 hours
Perform Ninhydrin test for general detection of amino acids.. Conduct Xanthoproteic acid test to identify aromatic amino acids.. Use Million's test to detect tyrosine and its derivatives..
- Week 4Module 1: pH Measurement and Qualitative Test
Unit 4: Qualitative Test for Lipid · 2 hours
Perform solubility tests to identify lipids based on their solubility in different solvents.. Conduct translucent spot test to confirm the presence of lipids.. Perform Emulsification test to detect the presence of lipids..
- Week 5Module 2: Quantitative Analysis and Chromatography
Unit 1: Quantitative Estimation of Carbohydrates · 2 hours
Learn the principles of Benedict's method for quantitative glucose estimation.. Prepare Benedict's reagent and perform titration.. Calculate the percentage of glucose in a solution..
- Week 6Module 2: Quantitative Analysis and Chromatography
Unit 2: Quantitative Estimation of Proteins · 2 hours
Perform Spectrophotometric estimation of protein content.. Prepare a standard curve using bovine serum albumin (BSA).. Estimate protein concentrations of unknown samples using the standard curve..
- Week 7Module 2: Quantitative Analysis and Chromatography
Unit 3: Quantitative Estimation of Lipids · 2 hours
Learn to determine the iodine value of lipids.. Perform titration to measure iodine absorption.. Calculate the iodine number to assess the degree of unsaturation..
- Week 8Module 2: Quantitative Analysis and Chromatography
Unit 4: Quantitative Estimation of Vitamins · 2 hours
Learn to test for Vitamin A using antimony trichloride.. Perform quantitative estimation of ascorbic acid (Vitamin C) in tablets.. Perform quantitative estimation of ascorbic acid (Vitamin C) in fruit juice..
- Week 9Module 2: Quantitative Analysis and Chromatography
Unit 5: Paper and Thin Layer Chromatography · 2 hours
Understand the principles of chromatography.. Perform separation of amino acids by paper chromatography.. Calculate Rf values to identify amino acids..
- Week 10Module 1: pH Measurement and Qualitative Test
Review of Module 1 · 2 hours
Review Module 1: pH Measurement and Qualitative Test. Practice pH measurement techniques.. Review qualitative tests for carbohydrates, amino acids, and lipids..
- Week 11Module 2: Quantitative Analysis and Chromatography
Review of Module 2 · 2 hours
Review Module 2: Quantitative Analysis and Chromatography. Practice quantitative estimation techniques.. Review paper and thin layer chromatography procedures..
- Week 12Module 1: pH Measurement and Qualitative Test
Assignments and TMA Preparation · 3 hours
Complete assignments related to pH measurement and qualitative tests.. Prepare for tutor-marked assignments (TMAs).. Review key concepts and procedures..
- Week 13Module 2: Quantitative Analysis and Chromatography
Assignments and TMA Preparation · 3 hours
Complete assignments related to quantitative analysis and chromatography.. Prepare for tutor-marked assignments (TMAs).. Review key concepts and procedures..
Preparing for the exam
- Review all practical procedures and understand the underlying principles.
- Practice calculations for quantitative estimations.
- Create flowcharts for each qualitative test to remember the steps and expected results.
- Focus on understanding the reactions involved in each test.
- Review all tutor-marked assignments (TMAs) and address any feedback.
- Practice thin layer chromatography techniques and calculations of Rf values.
Questions students ask about this course
What is EHS209 about?
This course provides Environmental Health Science students with practical knowledge of biochemistry. It covers pH determination, qualitative and quantitative tests for biomolecules like carbohydrates, amino acids, proteins, lipids, and vitamins. Students will also learn chromatographic separation techniques such as paper and thin layer chromatography, enabling them to perform essential biochemical analyses in the laboratory.
How many units does EHS209 have?
EHS209, General Biochemistry Practical Manual, has 9 units across 2 modules, over 74 pages of course material. You can read it one unit at a time.
How many credit units is EHS209?
EHS209 carries 1 credit unit, at 200 level in Health Sciences.
Is EHS209 hard?
EHS209 is rated intermediate level, with intermediate mathematical content. It is mostly practical and problem solving work, and it has a practical component.
How long does EHS209 take to study?
About 48 hours of study, spread across its 9 units.
How is EHS209 assessed?
EHS209 is assessed by assignments, tutor marked assessments and final examination.
What can I do with EHS209?
Environmental Health Officer, Laboratory Technician, Food Quality Control Analyst and Biomedical Scientist.