General Biochemistry Laboratory I
- Sciences
- 200 level
- 1 credit unit
- 56 pages
- 7 units
This course introduces students to the fundamental principles and practices of biochemistry laboratory work. It covers essential laboratory equipment, safety protocols, and accurate measurement techniques. Students will learn about photometry, colorimetry, pH and buffer systems, and qualitative/quantitative tests for amino acids and proteins. The course aims to equip students with the basic skills necessary for conducting biochemical research and investigations.
About this course
- Difficulty
- Intermediate
- Study hours
- 91 hours
- Maths
- Intermediate
- Content
- Practical, theoretical
- Practical work
- Yes
- Assignments
- Tutor Marked Assessments
- Final Examination
What you'll read
The real module and unit structure of BIO215, taken from the course material NOUN publishes.
- UNIT 1 INTRODUCTION TO LABORATORY AND LABORATORY EQUIPMENTPage 1
- UNIT 2 LABORATORY SAFETY CONTENTPage 6
- UNIT 3: ACCURACY OF MEASUREMENT AND TRANSFER OF LIQUIDS AND SOLIDS
- UNIT 4 INTRODUCTION TO PHOTOMETRY AND COLORIMETRYPage 26
- UNIT 5 STANDARD CURVE IN ABSORPTION SPECTRA
- UNIT 6 pH AND BUFFER SYSTEM
- UNIT 7 QUALITATIVE AND QUANTITATIVE TESTS FOR AMINO ACIDS AND PROTEINSPage 45
One paragraph, so you can see how it reads
BIO215 · UNIT 1 INTRODUCTION TO LABORATORY AND LABORATORY EQUIPMENT
Scientific laboratories can be found in schools and universities, in industry, in government or military facilities, and even aboard ships and spacecraft. A laboratory might offer work space for just one to more than thirty researchers depending on its size and purpose.
What you should be able to do
- Identify and use common biochemistry laboratory equipment.
- Apply safety protocols and procedures in the laboratory.
- Perform accurate measurements and calculations in biochemical experiments.
- Understand the principles of photometry and colorimetry.
- Prepare and use standard curves for quantitative analysis.
- Explain pH and buffer systems and their importance in biological systems.
- Perform qualitative and quantitative tests for amino acids and proteins.
What it prepares you for
- Laboratory Technician
- Research Assistant
- Biochemist
- Quality Control Analyst
- Pharmaceuticals
- Food Science
- Biotechnology
- Clinical Diagnostics
Where it gets hard
The units students slow down on, and what makes each one heavy.
- Module 2: Introduction to Photometry and Colorimetry
Unit 4: Introduction to Photometry and Colorimetry
Requires understanding of light absorption principles and complex calculations.
- Module 3: pH and Buffer System
Unit 6: pH and Buffer System
Involves logarithmic calculations and understanding of chemical equilibria.
A suggested way through it
13 weeks, about 35 hours in total. Yours will differ.
- Week 1Module 1: Introduction to Laboratory and Laboratory Equipment
Unit 1: Introduction to Laboratory and Laboratory Equipment · 2 hours
Read the introduction to understand the importance of laboratory work in scientific research.. Define the meaning of a laboratory and identify different types of laboratories.. List and identify different types of laboratory equipment and glassware..
- Week 2Module 1: Introduction to Laboratory and Laboratory Equipment
Unit 2: Laboratory Safety · 2 hours
Familiarize yourself with laboratory safety rules and precautions.. Understand the dos and don'ts of a biochemistry laboratory.. Learn about laboratory housekeeping and general cleaning tips..
- Week 3Module 2: Introduction to Photometry and Colorimetry
Unit 3: Accuracy of Measurement and Transfer of Liquids and Solids · 2 hours
Explain the importance of accuracy and precision in laboratory measurements.. Understand the different ways of expressing measurements in SI units.. Learn about potential sources of error in practical exercises..
- Week 4Module 2: Introduction to Photometry and Colorimetry
Unit 4: Introduction to Photometry and Colorimetry · 2 hours
Define spectrophotometry, colorimetry, and photometry.. Understand Beer-Lambert's law and its applications.. Perform a colorimetric estimation of inorganic phosphate..
- Week 5Module 3: pH and Buffer System
Unit 5: Standard Curve in Absorption Spectra · 2 hours
Explain the meaning of a standard curve and its use in quantitative analysis.. Learn how to prepare a standard curve using experimental data.. Practice using a standard curve to determine unknown concentrations..
- Week 6Module 3: pH and Buffer System
Unit 6: pH and Buffer System · 2 hours
Define pH and buffer solutions and understand their importance.. Learn how to calculate pH and prepare buffer solutions.. Understand the uses of pH and buffer solutions in biochemical experiments..
- Week 7Module 3: pH and Buffer System
Unit 7: Qualitative and Quantitative Tests for amino Acids and Proteins · 2 hours
Define proteins and amino acids and understand their roles in living systems.. Learn about qualitative tests for proteins and amino acids, such as the Ninhydrin and Biuret tests.. Understand the principles behind each test and their applications..
- Week 8Module 1: Introduction to Laboratory and Laboratory Equipment
Unit 1: Introduction to Laboratory and Laboratory Equipment · 2 hours
Review Module 1: Introduction to Laboratory and Laboratory Equipment. Focus on key concepts: laboratory safety, equipment identification, and measurement accuracy..
Unit 4: Introduction to Photometry and Colorimetry · 2 hours
Review Module 2: Introduction to Photometry and Colorimetry. Focus on key concepts: spectrophotometry, Beer-Lambert's law, and standard curve preparation..
- Week 9Module 3: pH and Buffer System
Unit 6: pH and Buffer System · 2 hours
Review Module 3: pH and Buffer System. Focus on key concepts: pH calculation, buffer preparation, and buffer systems in living organisms..
Unit 7: Qualitative and Quantitative Tests for amino Acids and Proteins · 2 hours
Review Module 3: pH and Buffer System. Focus on key concepts: Qualitative and quantitative tests for amino acids and proteins..
- Week 10Module 2: Introduction to Photometry and Colorimetry
Unit 3: Accuracy of Measurement and Transfer of Liquids and Solids · 3 hours
Practice problems related to accuracy of measurement and error analysis.. Complete a practical exercise on calibrating laboratory pipettes..
- Week 11Module 3: pH and Buffer System
Unit 4: Introduction to Photometry and Colorimetry · 3 hours
Perform a virtual lab experiment on spectrophotometry and colorimetry.. Analyze data and interpret results from the experiment..
- Week 12Module 2: Introduction to Photometry and Colorimetry
Unit 5: Standard Curve in Absorption Spectra · 3 hours
Practice preparing standard curves using different datasets.. Solve problems involving the determination of unknown concentrations using standard curves..
- Week 13Module 3: pH and Buffer System
Final Revision · 4 hours
Review all units and key concepts.. Work on assignments and prepare for final examination..
Preparing for the exam
- Review all laboratory procedures and safety protocols from Units 1 and 2.
- Practice calculations related to SI units, concentrations, and dilutions from Unit 3.
- Understand Beer-Lambert's Law and its applications in photometry and colorimetry (Unit 4).
- Practice preparing and interpreting standard curves (Unit 5).
- Master pH calculations and buffer preparation techniques (Unit 6).
- Review qualitative and quantitative tests for amino acids and proteins (Unit 7).
- Focus on understanding the principles behind each experiment and their applications.
- Solve all tutor-marked assignments and review feedback.
- Create flashcards for key terms and concepts.
- Allocate specific time slots for focused study and revision each week.
Questions students ask about this course
What is BIO215 about?
This course introduces students to the fundamental principles and practices of biochemistry laboratory work. It covers essential laboratory equipment, safety protocols, and accurate measurement techniques. Students will learn about photometry, colorimetry, pH and buffer systems, and qualitative/quantitative tests for amino acids and proteins. The course aims to equip students with the basic skills necessary for conducting biochemical research and investigations.
How many units does BIO215 have?
BIO215, General Biochemistry Laboratory I, has 7 units across 1 module, over 56 pages of course material. You can read it one unit at a time.
How many credit units is BIO215?
BIO215 carries 1 credit unit, at 200 level in Sciences.
Is BIO215 hard?
BIO215 is rated intermediate level, with intermediate mathematical content. It is mostly practical and theoretical work, and it has a practical component.
How long does BIO215 take to study?
About 91 hours of study, spread across its 7 units.
How is BIO215 assessed?
BIO215 is assessed by Assignments, Tutor Marked Assessments and Final Examination.
What can I do with BIO215?
Laboratory Technician, Research Assistant, Biochemist and Quality Control Analyst.