ANALYTICAL TECHNIQUES FOR ANIMAL PRODUCTION

1. Explain artificial insemination techniques and their significance in animal breeding.
2. Evaluate semen quality and apply appropriate storage methods.
3. Conduct and interpret digestibility trials using both in vivo and in vitro methods.
4. Perform and analyze biological assays to determine feed protein quality.
5. Apply chromatography and electrophoresis techniques for protein separation and characterization.
6. Utilize radio-isotopy and manometry in biological experiments.
7. Apply spectrometry and colorimetry techniques for quantitative analysis.
8. Prepare and purify enzymes for further analysis.

13 weeks, about 26 hours in total. Yours will differ.

1. Week 1Module 1: Artificial Insemination Techniques in Animal Breeding

   • Unit 1: Meaning of AI and Significance of AI · 2 hours

     Define artificial insemination (AI).. Differentiate between natural mating and AI.. Understand the importance of AI in animal breeding.. Identify the advantages and limitations of AI..

2. Week 2Module 1: Artificial Insemination Techniques in Animal Breeding

   • Unit 2: Semen collection techniques in farm animals · 2 hours

     Understand factors enhancing semen collection.. Learn methods of semen collection in farm animals.. Learn how to analyze semen on the farm and in the lab.. Identify the equipment for semen collection..

3. Week 3Module 1: Artificial Insemination Techniques in Animal Breeding

   • Unit 3: Evaluation of semen quality · 2 hours

     Identify parameters for semen evaluation.. Understand the significance of physical and biochemical properties indicating good semen quality.. Learn how to measure semen volume and sperm motility.. Understand the importance of live to dead ratio..

4. Week 4Module 1: Artificial Insemination Techniques in Animal Breeding

   • Unit 4 Techniques involved in the storage of collected semen · 2 hours

     Learn methods for semen storage.. Identify common semen diluents used for semen extension.. Understand factors influencing sperm cell survival during storage.. Differentiate between short-term and long-term storage..

5. Week 5Module 1: Artificial Insemination Techniques in Animal Breeding

   • Unit 5: Semen extension, insemination and factors that may affect fertility after insemination · 2 hours

     Understand reasons for extending semen samples.. Identify common semen diluents used for semen extension.. Learn the art of insemination.. Understand the factors that influence fertility during insemination..

6. Week 6Module 2: Digestibility trials (in vivo and in vitro)

   • Unit 6: An overview of digestibility trials · 2 hours

     Know the importance of digestibility trials.. Identify procedures to determine feed resources for livestock feeding.. Differentiate between in vivo and in vitro digestibility.. Understand the merits and limitations of both in vivo and in vitro trials..

7. Week 7Module 2: Digestibility trials (in vivo and in vitro)

   • Unit 7: Protocols for in vivo digestibility trials · 2 hours

     Understand techniques involved in conducting in vivo digestibility trials.. Learn about animal protocols and diet preparations.. Explore methods like direct collection, difference method, and regression method.. Learn how to calculate Dry Matter Digestibility (DMD)..

8. Week 8Module 2: Digestibility trials (in vivo and in vitro)

   • Unit 8: Protocols for in vitro digestibility trials · 2 hours

     Understand techniques involved in conducting in vitro digestibility trials.. Learn about Rumen fluid-pepsin in vitro digestibility (IVOMD).. Explore Enzyme-based assays.. Understand the limitations of in vitro digestibility trials..

9. Week 9Module 3: Biological assays involving PER, NPU, AD, ID and BV

   • Unit 9: Practical applications of biological assay · 2 hours

     Measure and observe livestock response in feeding experiments.. Understand the significance of biological assays.. Learn about Protein Efficiency Ratio (PER) and Net Protein Utilisation (NPU).. Differentiate between Apparent digestibility (AD) and True digestibility (TD)..

10. Week 10Module 4: Separation and characterisation of components involving the use of chromatography, electrophoresis, radio-isotopy, manometry, colorimetry, spectrometry

    • Unit 10: Techniques involved in separation and characterisation of components involving the use of chromatography and electrophoresis · 2 hours

      Understand techniques involved in protein separation.. Learn about chromatography and electrophoresis.. Explore column chromatography and High Performance Liquid Chromatography (HPLC).. Learn about separation of proteins using electrophoresis..

11. Week 11Module 4: Separation and characterisation of components involving the use of chromatography, electrophoresis, radio-isotopy, manometry, colorimetry, spectrometry

    • Unit 11: Techniques involved in separation and characterisation of components involving the use of radio-isotopy and manometry · 2 hours

      Understand the principle/application of radioisotopy to biological experiments.. Identify isotopes useful in agricultural research.. Learn about tracer techniques and autoradiography.. Understand the principle/application of manometry to biological experiments..

12. Week 12Module 4: Separation and characterisation of components involving the use of chromatography, electrophoresis, radio-isotopy, manometry, colorimetry, spectrometry

    • Unit 12: Techniques involved in separation and characterisation of components involving the use of spectrometry and colorimetry · 2 hours

      Understand the principle/application of spectrometry and colorimetry to biological experiments.. Learn about mass spectrometry and its components.. Explore the principles and application of colorimetry.. Understand Beer-Lambert law..

13. Week 13Module 4: Separation and characterisation of components involving the use of chromatography, electrophoresis, radio-isotopy, manometry, colorimetry, spectrometry

    • Unit 13: Determination, preparation and purification of enzymes · 2 hours

      Understand the properties and activities of enzymes.. Learn the process of extracting enzymes from tissues.. Understand the process of purifying enzymes from crude extract.. Learn about salt precipitation and dialysis..