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EHS209Health Sciences1 Unitsintermediate

General Biochemistry Practical Manual

This course provides Environmental Health Science students with practical knowledge of biochemistry. It covers pH determination, qualitative and quantitative tests for biomolecules like carbohydrates, amino acids, proteins, lipids, and vitamins. Students will also learn chromatographic separation techniques such as paper and thin layer chromatography, enabling them to perform essential biochemical analyses in the laboratory.

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48h
Study Time
13
Weeks
4h
Per Week
intermediate
Math Level
Course Keywords
BiochemistryPracticalEnvironmental HealthChromatographyQuantitative Analysis

Course Overview

Everything you need to know about this course

Course Difficulty

Intermediate Level
Builds on foundational knowledge
65%
intermediate
📊
Math Level
Moderate Math
🔬
Learning Type
Hands-on Practice

Course Topics

Key areas covered in this course

1

pH Measurement

2

Qualitative Tests for Carbohydrates

3

Qualitative Tests for Amino Acids

4

Qualitative Tests for Lipids

5

Quantitative Estimation of Carbohydrates

6

Quantitative Estimation of Proteins

7

Quantitative Estimation of Lipids

8

Quantitative Estimation of Vitamins

9

Paper Chromatography

10

Thin Layer Chromatography

Total Topics10 topics

Ready to Start

No specific requirements needed

This course is designed to be accessible to all students. You can start immediately without any prior knowledge or specific preparation.

Assessment Methods

How your progress will be evaluated (3 methods)

assignments

Comprehensive evaluation of course material understanding

Written Assessment

tutor-marked assessments

Comprehensive evaluation of course material understanding

Written Assessment

final examination

Comprehensive evaluation of course material understanding

Computer Based Test

Career Opportunities

Explore the career paths this course opens up for you

Environmental Health Officer

Apply your skills in this growing field

Laboratory Technician

Apply your skills in this growing field

Food Quality Control Analyst

Apply your skills in this growing field

Biomedical Scientist

Apply your skills in this growing field

Industry Applications

Real-world sectors where you can apply your knowledge

Food IndustryPharmaceutical IndustryEnvironmental MonitoringClinical Laboratories

Study Schedule Beta

A structured 13-week journey through the course content

Week
1

Module 1: pH Measurement and Qualitative Test

2h

Unit 1: Measurement of pH of solutions

2 study hours
  • Understand the pH scale and its significance.
  • Learn to use pH paper and pH meters for accurate measurements.
  • Conduct experiments to determine the pH of various solutions.
Week
2

Module 1: pH Measurement and Qualitative Test

2h

Unit 2: Qualitative Test for Carbohydrates

2 study hours
  • Perform Molisch's test to detect the presence of carbohydrates.
  • Conduct Fehling's test to identify reducing sugars.
  • Use Barfoed's test to differentiate between monosaccharides and disaccharides.
Week
3

Module 1: pH Measurement and Qualitative Test

2h

Unit 3: Qualitative Test for Amino Acid

2 study hours
  • Perform Ninhydrin test for general detection of amino acids.
  • Conduct Xanthoproteic acid test to identify aromatic amino acids.
  • Use Million's test to detect tyrosine and its derivatives.
Week
4

Module 1: pH Measurement and Qualitative Test

2h

Unit 4: Qualitative Test for Lipid

2 study hours
  • Perform solubility tests to identify lipids based on their solubility in different solvents.
  • Conduct translucent spot test to confirm the presence of lipids.
  • Perform Emulsification test to detect the presence of lipids.
Week
5

Module 2: Quantitative Analysis and Chromatography

2h

Unit 1: Quantitative Estimation of Carbohydrates

2 study hours
  • Learn the principles of Benedict's method for quantitative glucose estimation.
  • Prepare Benedict's reagent and perform titration.
  • Calculate the percentage of glucose in a solution.
Week
6

Module 2: Quantitative Analysis and Chromatography

2h

Unit 2: Quantitative Estimation of Proteins

2 study hours
  • Perform Spectrophotometric estimation of protein content.
  • Prepare a standard curve using bovine serum albumin (BSA).
  • Estimate protein concentrations of unknown samples using the standard curve.
Week
7

Module 2: Quantitative Analysis and Chromatography

2h

Unit 3: Quantitative Estimation of Lipids

2 study hours
  • Learn to determine the iodine value of lipids.
  • Perform titration to measure iodine absorption.
  • Calculate the iodine number to assess the degree of unsaturation.
Week
8

Module 2: Quantitative Analysis and Chromatography

2h

Unit 4: Quantitative Estimation of Vitamins

2 study hours
  • Learn to test for Vitamin A using antimony trichloride.
  • Perform quantitative estimation of ascorbic acid (Vitamin C) in tablets.
  • Perform quantitative estimation of ascorbic acid (Vitamin C) in fruit juice.
Week
9

Module 2: Quantitative Analysis and Chromatography

2h

Unit 5: Paper and Thin Layer Chromatography

2 study hours
  • Understand the principles of chromatography.
  • Perform separation of amino acids by paper chromatography.
  • Calculate Rf values to identify amino acids.
Week
10

Module 1: pH Measurement and Qualitative Test

2h

Review of Module 1

2 study hours
  • Review Module 1: pH Measurement and Qualitative Test
  • Practice pH measurement techniques.
  • Review qualitative tests for carbohydrates, amino acids, and lipids.
Week
11

Module 2: Quantitative Analysis and Chromatography

2h

Review of Module 2

2 study hours
  • Review Module 2: Quantitative Analysis and Chromatography
  • Practice quantitative estimation techniques.
  • Review paper and thin layer chromatography procedures.
Week
12

Module 1: pH Measurement and Qualitative Test

3h

Assignments and TMA Preparation

3 study hours
  • Complete assignments related to pH measurement and qualitative tests.
  • Prepare for tutor-marked assignments (TMAs).
  • Review key concepts and procedures.
Week
13

Module 2: Quantitative Analysis and Chromatography

3h

Assignments and TMA Preparation

3 study hours
  • Complete assignments related to quantitative analysis and chromatography.
  • Prepare for tutor-marked assignments (TMAs).
  • Review key concepts and procedures.

This study schedule is in beta and may not be accurate. Please use it as a guide and consult the course outline for the most accurate information.

Course PDF Material

Read the complete course material as provided by NOUN.

Access PDF Material

Study Tips & Exam Preparation

Expert tips to help you succeed in this course

1

Review all practical procedures and understand the underlying principles.

2

Practice calculations for quantitative estimations.

3

Create flowcharts for each qualitative test to remember the steps and expected results.

4

Focus on understanding the reactions involved in each test.

5

Review all tutor-marked assignments (TMAs) and address any feedback.

6

Practice thin layer chromatography techniques and calculations of Rf values.

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