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ANP309Agricultural Sciences2 Unitsintermediate

ANALYTICAL TECHNIQUES FOR ANIMAL PRODUCTION II

This course introduces students to analytical techniques used in animal production. It covers artificial insemination techniques, semen collection and evaluation, and semen storage. The course also explores digestibility trials, including in vivo and in vitro methods. Furthermore, it examines biological assays such as PER, NPU, AD, ID, and BV. Finally, the course delves into separation and characterization techniques like chromatography, electrophoresis, radio-isotopy, manometry, colorimetry, and spectrometry.

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156h
Study Time
13
Weeks
12h
Per Week
intermediate
Math Level
Course Keywords
Artificial InseminationDigestibility TrialsBiological AssaysChromatographySpectrometry

Course Overview

Everything you need to know about this course

Course Difficulty

Intermediate Level
Builds on foundational knowledge
65%
intermediate
📊
Math Level
Moderate Math
🔬
Learning Type
Hands-on Practice

Course Topics

Key areas covered in this course

1

Artificial Insemination Techniques

2

Semen Collection and Evaluation

3

Semen Storage

4

Digestibility Trials (In vivo and In vitro)

5

Biological Assays (PER, NPU, AD, ID, BV)

6

Chromatography and Electrophoresis

7

Radio-isotopy and Manometry

8

Spectrometry and Colorimetry

9

Enzyme Preparation and Purification

Total Topics9 topics

Requirements

Knowledge and skills recommended for success

Basic knowledge of animal biology

Basic chemistry concepts

💡 Don't have all requirements? Don't worry! Many students successfully complete this course with basic preparation and dedication.

Assessment Methods

How your progress will be evaluated (2 methods)

Tutor Marked Assignments

Comprehensive evaluation of course material understanding

Written Assessment

Final Examination

Comprehensive evaluation of course material understanding

Written Assessment

Career Opportunities

Explore the career paths this course opens up for you

Animal Breeder

Apply your skills in this growing field

Livestock Manager

Apply your skills in this growing field

Animal Nutritionist

Apply your skills in this growing field

Research Scientist

Apply your skills in this growing field

Laboratory Technician

Apply your skills in this growing field

Industry Applications

Real-world sectors where you can apply your knowledge

Animal HusbandryAgricultural ResearchFood SciencePharmaceuticalsBiotechnology

Study Schedule Beta

A structured 13-week journey through the course content

Week
1

MODULE ONE: ARTIFICIAL INSEMINATION TECHNIQUES IN ANIMAL BREEDING

2h

Unit 1: Meaning of AI and Significance of AI

2 study hours
  • Define artificial insemination (AI).
  • Differentiate between natural mating and AI.
  • Understand the importance of AI in animal breeding.
  • Identify advantages and limitations of AI.
Week
2

MODULE ONE: ARTIFICIAL INSEMINATION TECHNIQUES IN ANIMAL BREEDING

2h

Unit 2: Semen collection techniques in farm animals

2 study hours
  • Understand factors enhancing semen collection.
  • Learn methods of semen collection in farm animals.
  • Learn how to analyze semen on the farm and in the lab.
  • Understand the Aspiration, Massage, Electroejaculator, Gloved-hand, and Artificial vagina techniques.
Week
3

MODULE ONE: ARTIFICIAL INSEMINATION TECHNIQUES IN ANIMAL BREEDING

2h

Unit 3: Evaluation of semen quality

2 study hours
  • Identify physical variables for semen evaluation: color, volume, motility.
  • Learn to evaluate semen quality using microscopic techniques.
  • Understand the importance of sperm morphology and concentration.
  • Perform live/dead sperm ratio tests.
Week
4

MODULE ONE: ARTIFICIAL INSEMINATION TECHNIQUES IN ANIMAL BREEDING

2h

Unit 4 Techniques involved in the storage of collected semen

2 study hours
  • Learn methods for semen storage: short-term and long-term.
  • Identify common semen diluents and their functions.
  • Understand factors influencing sperm cell survival during storage.
  • Explore types of storage methods for frozen semen.
Week
5

MODULE ONE: ARTIFICIAL INSEMINATION TECHNIQUES IN ANIMAL BREEDING

2h

Unit 5: Semen extension, insemination and factors that may affect fertility after insemination

2 study hours
  • Understand reasons for extending semen samples.
  • Learn about common semen diluents and their characteristics.
  • Study the art of insemination techniques.
  • Explore factors influencing fertility during insemination.
Week
6

Module 2: Digestibility trials (*in vivo* and *in vitro*)

2h

Unit 6: An overview of digestibility trials

2 study hours
  • Define digestibility and digestibility trials.
  • Understand the significance of digestibility trials.
  • Differentiate between in vivo and in vitro digestibility.
  • Compare merits and limitations of in vivo and in vitro trials.
Week
7

Module 2: Digestibility trials (*in vivo* and *in vitro*)

2h

Unit 7: Protocols for *in vivo* digestibility trials

2 study hours
  • Understand techniques involved in conducting in vivo digestibility trials.
  • Learn about animal protocols and diet preparations.
  • Explore methods of in vivo digestibility: direct collection, difference method, regression method.
  • Calculate digestibility parameters.
Week
8

Module 2: Digestibility trials (*in vivo* and *in vitro*)

2h

Unit 8: Protocols for *in vitro* digestibility trials

2 study hours
  • Understand techniques involved in conducting in vitro digestibility trials.
  • Learn about Rumen fluid-pepsin in vitro digestibility (IVOMD).
  • Study Rumen fluid-Neutral detergent methods.
  • Explore Enzyme-based assays.
Week
9

Module 3: Biological assays involving PER, NPU, AD, ID and BV

2h

Unit 9: Practical applications of biological assay

2 study hours
  • Understand the significance of biological assays.
  • Explore types of biological assays: PER, NPU, AD, TD, BV.
  • Learn to measure and observe livestock response in feeding experiments.
  • Calculate protein efficiency ratio (PER).
Week
10

Module 4: Separation and characterisation of components involving the use of chromatography, electrophoresis, radio-isotopy, manometry, colorimetry, spectrometry

2h

Unit 10: Techniques involved in separation and characterisation of components involving the use of chromatography and electrophoresis

2 study hours
  • Understand techniques for separation of proteins.
  • Learn about chromatography: column, HPLC, adsorption, ion exchange, hydrophobic, gel filtration, affinity.
  • Study separation of proteins using electrophoresis: agarose gel, polyacrylamide gel.
  • Understand the principles of each technique.
Week
11

Module 4: Separation and characterisation of components involving the use of chromatography, electrophoresis, radio-isotopy, manometry, colorimetry, spectrometry

2h

Unit 11: Techniques involved in separation and characterisation of components involving the use of radio-isotopy and manometry

2 study hours
  • Understand the principle/application of radioisotopy to biological experiments.
  • Learn about the use of radioisotopy in agriculture.
  • Understand the principle/application of manometry to biological experiments.
  • Explore tracer techniques and autoradiography.
Week
12

Module 4: Separation and characterisation of components involving the use of chromatography, electrophoresis, radio-isotopy, manometry, colorimetry, spectrometry

2h

Unit 12: Techniques involved in separation and characterisation of components involving the use of spectrometry and colorimetry

2 study hours
  • Understand the principle/application of spectrometry to biological experiments.
  • Learn about the principles and applications of colorimetry.
  • Explore mass spectrometry and its components.
  • Study the Beer-Lambert law and its application in colorimetry.
Week
13

Module 4: Separation and characterisation of components involving the use of chromatography, electrophoresis, radio-isotopy, manometry, colorimetry, spectrometry

2h

Unit 13: Determination, preparation and purification of enzymes

2 study hours
  • Understand the properties and activities of enzymes.
  • Learn the process of extracting enzymes from tissues.
  • Understand the process of purifying enzymes from crude extract.
  • Explore the mode of action of enzymes.

This study schedule is in beta and may not be accurate. Please use it as a guide and consult the course outline for the most accurate information.

Course PDF Material

Read the complete course material as provided by NOUN.

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Study Tips & Exam Preparation

Expert tips to help you succeed in this course

1

Review definitions and applications of artificial insemination techniques (Units 1-5).

2

Practice calculations for digestibility trials and biological assays (Units 6-9).

3

Create concept maps linking chromatography, electrophoresis, spectrometry, and colorimetry (Units 10-12).

4

Focus on enzyme properties, extraction, and purification methods (Unit 13).

5

Study key differences between in vivo and in vitro methods, and their applications (Units 6-8).

6

Review all Tutor Marked Assignments (TMAs) and focus on areas where you had difficulty.

7

Allocate specific study time for each module, prioritizing challenging units.

8

Practice applying theoretical concepts to real-world scenarios in animal production.

9

Create flashcards for key terms and definitions in analytical techniques.

10

Form a study group to discuss and clarify complex concepts.

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