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BIO218Sciences2 Unitsintermediate

General Biochemistry Laboratory II

This course introduces students to general biochemistry laboratory practices. It covers topics such as concentration calculations, carbohydrate reactions, and chromatographic separation of sugars. Students will learn to estimate glucose in biological fluids, analyze lipids, and separate/purify nucleic acids. The course also includes estimation of phosphate and titratable acidity, providing a foundational understanding of biochemical laboratory techniques.

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40h
Study Time
13
Weeks
3h
Per Week
basic
Math Level
Course Keywords
BiochemistryLaboratory TechniquesCarbohydratesLipidsNucleic Acids

Course Overview

Everything you need to know about this course

Course Difficulty

Intermediate Level
Builds on foundational knowledge
65%
intermediate
➕
Math Level
Basic Math
🔬
Learning Type
Hands-on Practice

Course Topics

Key areas covered in this course

1

Concentration Calculations

2

Carbohydrate Reactions

3

Chromatography

4

Glucose Estimation

5

Lipid Analysis

6

Nucleic Acid Separation

7

Phosphate and Titratable Acidity

Total Topics7 topics

Ready to Start

No specific requirements needed

This course is designed to be accessible to all students. You can start immediately without any prior knowledge or specific preparation.

Assessment Methods

How your progress will be evaluated (3 methods)

assignments

Comprehensive evaluation of course material understanding

Written Assessment

laboratory reports

Comprehensive evaluation of course material understanding

Written Assessment

final examination

Comprehensive evaluation of course material understanding

Computer Based Test

Career Opportunities

Explore the career paths this course opens up for you

Biochemist

Apply your skills in this growing field

Laboratory Technician

Apply your skills in this growing field

Research Assistant

Apply your skills in this growing field

Quality Control Analyst

Apply your skills in this growing field

Industry Applications

Real-world sectors where you can apply your knowledge

PharmaceuticalsFood ScienceClinical DiagnosticsBiotechnology

Study Schedule Beta

A structured 13-week journey through the course content

Week
1

Module 1: General Texts in Concentration

2h

Unit 1: General Texts in Concentration

2 study hours
  • •Understand molarity, molality, and normality.
  • •Practice calculations for preparing solutions of different concentrations.
Week
2

Module 2: Reactions of Carbohydrate, Thin Layer of Chromatographic Separation of Sugar

2h

Unit 2: Reactions of Carbohydrate, Thin Layer of Chromatographic Separation of Sugar

2 study hours
  • •Perform Molisch, Fehling's, Barfoed's, and Benedict's tests.
  • •Observe color changes and interpret results.
Week
3

Module 2: Reactions of Carbohydrate, Thin Layer of Chromatographic Separation of Sugar

2h

Unit 2: Reactions of Carbohydrate, Thin Layer of Chromatographic Separation of Sugar

2 study hours
  • •Prepare TLC plates and spot sugar samples.
  • •Develop chromatogram and calculate Rf values.
Week
4

Module 3: Estimation of Glucose in Biological Fluid (Blood and Urine)

2h

Unit 3: Estimation of Glucose in Biological Fluid (Blood and Urine)

2 study hours
  • •Perform Folin-Wu method for glucose estimation.
  • •Compare results with standard glucose solutions.
Week
5

Module 3: Estimation of Glucose in Biological Fluid (Blood and Urine)

2h

Unit 3: Estimation of Glucose in Biological Fluid (Blood and Urine)

2 study hours
  • •Perform Benedict's titration to estimate sugar in urine.
  • •Adjust urine dilution as necessary.
Week
6

Module 4: Analysis of Lipids for Double Bond and Free Fatty Acids Contents

2h

Unit 4: Analysis of Lipids for Double Bond and Free Fatty Acids Contents

2 study hours
  • •Determine iodine number of a lipid sample.
  • •Compare results with known standards.
Week
7

Module 5: Separation of Lipids by Thin Layer Chromatography

2h

Unit 5: Separation of Lipids by Thin Layer Chromatography

2 study hours
  • •Prepare TLC plates and spot lipid samples.
  • •Develop chromatogram and identify lipid fractions.
Week
8

Module 6: Separation and Purification of Nucleic Acids

2h

Unit 6: Separation and Purification of Nucleic Acids

2 study hours
  • •Extract RNA from yeast using phenol.
  • •Precipitate RNA with alcohol.
Week
9

Module 6: Separation and Purification of Nucleic Acids

2h

Unit 6: Separation and Purification of Nucleic Acids

2 study hours
  • •Isolate DNA from pig spleen using buffered saline.
  • •Remove protein with chloroform/amyl alcohol mixture.
Week
10

Module 6: Separation and Purification of Nucleic Acids

2h

Unit 6: Separation and Purification of Nucleic Acids

2 study hours
  • •Estimate DNA concentration using diphenylamine reaction.
  • •Measure absorbance at 595 nm.
Week
11

Module 7: Estimation of Phosphate and Titratable Acidity Content

2h

Unit 7: Estimation of Phosphate and Titratable Acidity Content

2 study hours
  • •Measure urine pH using a pH meter.
  • •Estimate phosphate content using molybdic acid and ascorbate.
Week
12

Module 7: Estimation of Phosphate and Titratable Acidity Content

2h

Unit 7: Estimation of Phosphate and Titratable Acidity Content

2 study hours
  • •Titrate urine with standardized NaOH to determine titratable acidity.
  • •Observe color change using phenolphthalein indicator.
Week
13

Final Revision

4h

Review of all units

4 study hours
  • •Review all units and laboratory procedures.
  • •Practice calculations and techniques.

This study schedule is in beta and may not be accurate. Please use it as a guide and consult the course outline for the most accurate information.

Course PDF Material

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Study Tips & Exam Preparation

Expert tips to help you succeed in this course

1

Review concentration calculations from Unit 1 and practice similar problems.

2

Create concept maps linking carbohydrate reactions from Unit 2.

3

Practice TLC techniques from Units 2 and 5.

4

Understand the principles behind glucose estimation methods in Unit 3.

5

Review the steps involved in nucleic acid separation from Unit 6.

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