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BIO215Sciences1 Unitsintermediate

General Biochemistry Laboratory I

This course introduces students to the fundamental principles and practices of biochemistry laboratory work. It covers essential laboratory equipment, safety protocols, and accurate measurement techniques. Students will learn about photometry, colorimetry, pH and buffer systems, and qualitative/quantitative tests for amino acids and proteins. The course aims to equip students with the basic skills necessary for conducting biochemical research and investigations.

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91h
Study Time
13
Weeks
7h
Per Week
intermediate
Math Level
Course Keywords
BiochemistryLaboratory TechniquesPhotometryAmino AcidsProteins

Course Overview

Everything you need to know about this course

Course Difficulty

Intermediate Level
Builds on foundational knowledge
65%
intermediate
📊
Math Level
Moderate Math
🔬
Learning Type
Hands-on Practice

Course Topics

Key areas covered in this course

1

Laboratory Equipment

2

Laboratory Safety

3

Measurement Accuracy

4

Photometry and Colorimetry

5

Standard Curves

6

pH and Buffer Systems

7

Amino Acids and Proteins

Total Topics7 topics

Ready to Start

No specific requirements needed

This course is designed to be accessible to all students. You can start immediately without any prior knowledge or specific preparation.

Assessment Methods

How your progress will be evaluated (3 methods)

Assignments

Comprehensive evaluation of course material understanding

Written Assessment

Tutor-Marked Assessments

Comprehensive evaluation of course material understanding

Written Assessment

Final Examination

Comprehensive evaluation of course material understanding

Computer Based Test

Career Opportunities

Explore the career paths this course opens up for you

Laboratory Technician

Apply your skills in this growing field

Research Assistant

Apply your skills in this growing field

Biochemist

Apply your skills in this growing field

Quality Control Analyst

Apply your skills in this growing field

Industry Applications

Real-world sectors where you can apply your knowledge

PharmaceuticalsFood ScienceBiotechnologyClinical Diagnostics

Study Schedule Beta

A structured 13-week journey through the course content

Week
1

Module 1: Introduction to Laboratory and Laboratory Equipment

2h

Unit 1: Introduction to Laboratory and Laboratory Equipment

2 study hours
  • •Read the introduction to understand the importance of laboratory work in scientific research.
  • •Define the meaning of a laboratory and identify different types of laboratories.
  • •List and identify different types of laboratory equipment and glassware.
Week
2

Module 1: Introduction to Laboratory and Laboratory Equipment

2h

Unit 2: Laboratory Safety

2 study hours
  • •Familiarize yourself with laboratory safety rules and precautions.
  • •Understand the dos and don'ts of a biochemistry laboratory.
  • •Learn about laboratory housekeeping and general cleaning tips.
Week
3

Module 2: Introduction to Photometry and Colorimetry

2h

Unit 3: Accuracy of Measurement and Transfer of Liquids and Solids

2 study hours
  • •Explain the importance of accuracy and precision in laboratory measurements.
  • •Understand the different ways of expressing measurements in SI units.
  • •Learn about potential sources of error in practical exercises.
Week
4

Module 2: Introduction to Photometry and Colorimetry

2h

Unit 4: Introduction to Photometry and Colorimetry

2 study hours
  • •Define spectrophotometry, colorimetry, and photometry.
  • •Understand Beer-Lambert's law and its applications.
  • •Perform a colorimetric estimation of inorganic phosphate.
Week
5

Module 3: pH and Buffer System

2h

Unit 5: Standard Curve in Absorption Spectra

2 study hours
  • •Explain the meaning of a standard curve and its use in quantitative analysis.
  • •Learn how to prepare a standard curve using experimental data.
  • •Practice using a standard curve to determine unknown concentrations.
Week
6

Module 3: pH and Buffer System

2h

Unit 6: pH and Buffer System

2 study hours
  • •Define pH and buffer solutions and understand their importance.
  • •Learn how to calculate pH and prepare buffer solutions.
  • •Understand the uses of pH and buffer solutions in biochemical experiments.
Week
7

Module 3: pH and Buffer System

2h

Unit 7: Qualitative and Quantitative Tests for amino Acids and Proteins

2 study hours
  • •Define proteins and amino acids and understand their roles in living systems.
  • •Learn about qualitative tests for proteins and amino acids, such as the Ninhydrin and Biuret tests.
  • •Understand the principles behind each test and their applications.
Week
8

Module 1: Introduction to Laboratory and Laboratory Equipment

4h

Unit 1: Introduction to Laboratory and Laboratory Equipment

2 study hours
  • •Review Module 1: Introduction to Laboratory and Laboratory Equipment
  • •Focus on key concepts: laboratory safety, equipment identification, and measurement accuracy.

Unit 4: Introduction to Photometry and Colorimetry

2 study hours
  • •Review Module 2: Introduction to Photometry and Colorimetry
  • •Focus on key concepts: spectrophotometry, Beer-Lambert's law, and standard curve preparation.
Week
9

Module 3: pH and Buffer System

4h

Unit 6: pH and Buffer System

2 study hours
  • •Review Module 3: pH and Buffer System
  • •Focus on key concepts: pH calculation, buffer preparation, and buffer systems in living organisms.

Unit 7: Qualitative and Quantitative Tests for amino Acids and Proteins

2 study hours
  • •Review Module 3: pH and Buffer System
  • •Focus on key concepts: Qualitative and quantitative tests for amino acids and proteins.
Week
10

Module 2: Introduction to Photometry and Colorimetry

3h

Unit 3: Accuracy of Measurement and Transfer of Liquids and Solids

3 study hours
  • •Practice problems related to accuracy of measurement and error analysis.
  • •Complete a practical exercise on calibrating laboratory pipettes.
Week
11

Module 3: pH and Buffer System

3h

Unit 4: Introduction to Photometry and Colorimetry

3 study hours
  • •Perform a virtual lab experiment on spectrophotometry and colorimetry.
  • •Analyze data and interpret results from the experiment.
Week
12

Module 2: Introduction to Photometry and Colorimetry

3h

Unit 5: Standard Curve in Absorption Spectra

3 study hours
  • •Practice preparing standard curves using different datasets.
  • •Solve problems involving the determination of unknown concentrations using standard curves.
Week
13

Module 3: pH and Buffer System

4h

Final Revision

4 study hours
  • •Review all units and key concepts.
  • •Work on assignments and prepare for final examination.

This study schedule is in beta and may not be accurate. Please use it as a guide and consult the course outline for the most accurate information.

Course PDF Material

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Study Tips & Exam Preparation

Expert tips to help you succeed in this course

1

Review all laboratory procedures and safety protocols from Units 1 and 2.

2

Practice calculations related to SI units, concentrations, and dilutions from Unit 3.

3

Understand Beer-Lambert's Law and its applications in photometry and colorimetry (Unit 4).

4

Practice preparing and interpreting standard curves (Unit 5).

5

Master pH calculations and buffer preparation techniques (Unit 6).

6

Review qualitative and quantitative tests for amino acids and proteins (Unit 7).

7

Focus on understanding the principles behind each experiment and their applications.

8

Solve all tutor-marked assignments and review feedback.

9

Create flashcards for key terms and concepts.

10

Allocate specific time slots for focused study and revision each week.

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